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Image Search Results
Journal: Anemia
Article Title: Sickle Cell Anemia Screening in Newborns and Analysis of Haplotypes in Patients from Santiago Island, Cape Verde
doi: 10.1155/2024/1687917
Figure Lengend Snippet: Results of gel electrophoresis of PCR-RFLP products amplified with beta-globin primers and digested with DdeI enzyme, visualized under UV light. Lane 1: 100 bp DNA ladder. Lanes 2–15: samples amplified with S1/S2 beta-globin primers and digested with DdeI enzyme. Lane 16: positive control. Lane 17: negative control. Lane 18: 100 bp DNA ladder. Interpretation: samples 1–11 exhibit amplification with beta-globin primers and partial digestion with DdeI enzyme. Samples 12, 13, and 14 show amplification with beta-globin primers and complete digestion with DdeI enzyme, indicating that the infants are negative for SCA.
Article Snippet: Digestion of S1/S2 amplified products was carried out with the
Techniques: Nucleic Acid Electrophoresis, Amplification, Positive Control, Negative Control